Analysis of Agrobacterium strain which has unique characterisitics suitable for rice transformation

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580115
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Hiroshima University
• Principal Investigator: SUZUKI Katsunori 広島大学, 理学(系)研究科(研究院), 教授 (50221320)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Shinji 広島大学, 理学(系)研究科(研究院), 助教 (50607348)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: アグロバクテリア / イネ / 形質転換 / 感染遺伝子発現 / 遺伝子発現誘導物質

Outline of Final Research Achievements

The primary aim of this study is to reveal the mechanism of a unique Agrobacterium strain that has property suitable for rice transformation. So far, they thought that rice has no or little material that induces vir gene expression in agrobacteria. Here we successfully extract and purify the substance, and then determined the molecular structure. The fact was supported by an experiment using the chemically synthesized material. This study also showed that the phenotype of the strain is determined not by Ti plasmid but by chromosomal background of the strain.

Free Research Field

微生物学、分子遺伝学