2014 Fiscal Year Final Research Report
Investigation of a novel transcriptional repression mechanism of PCB degradation genes
| Project/Area Number |
24580121
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Tohoku Gakuin University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 転写制御 / カタボライト抑制 / 細菌 / Rhodococcus / ポリ塩化ビフェニル |
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| Outline of Final Research Achievements |
In Rhodococcus jostii RHA1, known as a polychlorinated biphenyl (PCB) degrader, the transcription of biphenyl degradation genes is up-regulated in the presence of biphenyl, while it is repressed by the coexistence of benzoate which is one of the intermediate compounds of biphenyl degradation. In this study, the mechanism of this repression was investigated. It was revealed that it is catechol, a downstream compound of benzoate degradation, that causes the repression of the transcription of biphenyl degradation genes, and the overexpression of the catechol degradation gene (catA) in RHA1 canceled the repression and enhanced the growth of RHA1 on biphenyl. Genes which have been known to be responsible for the catabolite repression, crp, was proved not to participate in this repression.
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| Free Research Field |
分子微生物学
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