Piezo-adaptation of deep-sea microbes analyzed by measurements of the membrane dynamics

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580122
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Aoyama Gakuin University
• Principal Investigator: ABE Fumiyoshi 青山学院大学, 理工学部, 教授 (30360746)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 深海好圧性細菌 / 細胞膜 / 膜物性 / 時間分解蛍光偏光解消法 / 高圧適応

Outline of Final Research Achievements

Deep-sea piezophiles have adapted to high-pressure environments. The cell membrane is important for substrate transports and energy production, and its functions are generally sensitive to high pressure. This study investigates the microbial adaptation to high pressurein terms of membrane dynamics. Deep-sea isolates of Shewanella, Moritella and Photobacterim were subjected to time-resolved anisotropy measurement using TMA-DPH as a polarization probe to measure the membrane order and the rotational motion of the acyl chains. I found that bacterial strains isoalted from deeper habitats had more rigid cell membrane. This view is distinct from common belief that deep-sea microbes have more fluid cell membrane.

Free Research Field

分子遺伝学