Biochemical and genetic analysis of the methylation-promoting factor for the RsmG methyltransferase

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580131
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: OKAMOTO Susumu 独立行政法人農業・食品産業技術総合研究機構, 食品総合研究所食品バイオテクノロジー研究領域, 上席研究員 (80353986)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: RNA / 塩基修飾 / メチル化酵素

Outline of Final Research Achievements

Bacterial rRNAs have many modified nucleotides. Among them, a 7-methylguanosine (m7G) modification in 16S rRNA is highly conserved among eubacteria. This m7G modification is catalyzed by the RsmG methyltransferase. In Escherichia coli, the methylation reaction catalyzed by RsmG is significantly promoted by an unknown protein factor. Here, we conducted biochemical and genetic analyses of this methylation-promoting factor. We have identified the Tas protein as a candidate.

Free Research Field

微生物学