Characterization of novel yeast transporter and improvement of method for measurement of organelle ion transporter

2015 Fiscal Year Final Research Report

• Project/Area Number: 24580135
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Tohoku University
• Principal Investigator: Hamamoto Shin 東北大学, 工学(系)研究科(研究院), 助教 (10533812)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: イオンチャネル

Outline of Final Research Achievements

YVC1 is a major cation channel localized in yeast vacuole membrane, and it is activated by cytoplasmic Ca2+. We have applied the patch-clamp technique using enlarged yeast cells to elucidate the regulatory mechanisms of the YVC1-mediated channel activity. We have tested the addition of reducing agent into the buffer corresponding to the cytosolic side. Moreover, we have identified the cysteine crucial for the functional property of the YVC1 channel in the presence of thiol group chemicals. And also we measured the single-channel conductance of both of the wild-type YVC1 channel and serine substituted YVC1 channel. The both channels have consistent conductance 300 pS, which suggested that substitution to serine did not affect to the channel conductance. We examined the effect of inositol phosphate on TPC2 channel expressed in yeast vacuole membrane.

Free Research Field

生化学