2014 Fiscal Year Final Research Report
Studies on the enzymes related to central metabolism of hyperthermophilic archaea
Project/Area Number |
24580136
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Applied biochemistry
|
Research Institution | The University of Tokyo |
Principal Investigator |
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Keywords | 酵素 / 酸化還元酵素 / 古細菌 / 超好熱菌 / フェレドキシン / アロステリズム / 中央代謝 / 結晶構造解析 |
Outline of Final Research Achievements |
Structure-function relationships of several enzymes related to central metabolism of acidophilic and hyperthermophilic archaeon were investigated. Glyceraldehyde-3-phosphate dehydrogenases including traditional phosphorylating type GAPDH and non-phosphrylating enzyme GAPN were characterized. Single amino acid substitution of GAPN resulted in conversion of the allosterism type from K into V. Three isozymes of glyceraldehyde oxidoreductase related to glycolysis were isolated. They showed novel subunit compositions and the crystal structure suggested characteristic molecular construction pathway. 2-Oxoacid:ferredoxin oxidoreductase from the organism has only one iron-sulfur clusted ligated by 4 Cys. Substitution of Cys and X-ray crystallography suggested a novel reaction mechanism. Ferredoxin NADP(+) reductase was identified by analyzing ST2133.
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Free Research Field |
酵素学
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