2014 Fiscal Year Final Research Report
Functional analysis and molecular basis of leucine as a biological regulator
Project/Area Number |
24580137
|
Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Applied biochemistry
|
Research Institution | The University of Tokyo |
Principal Investigator |
TOMITA Takeo 東京大学, 生物生産工学研究センター, 助教 (50447364)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Keywords | ロイシン / 生体調節因子 / 好熱菌 / グルタミン酸脱水素酵素 / シグナル伝達 / アロステリック調節 / グローバル調節 / タンパク質間相互作用 |
Outline of Final Research Achievements |
Molecular partner of SraA, which was supposed to be a sensor of amino acid, was searched and we identified TrpD, a second enzyme on biosynthesis of tryptophan. We performed biochemical analyses and suggested that SraA sensed tryptophan, which was an end product of the biosynthetic pathway, and transmitted the signal toward TrpD to inhibit the activity. This is a novel mechanism for feedback inhibition of tryptophan biosynthetic pathway. We also found GDH interacted with a homolog of enzyme involved in purine biosynthetic pathway and indicated existence of a novel regulatory mechanism of intracellular metabolism.
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Free Research Field |
構造生物学
|