2014 Fiscal Year Final Research Report
Analysis of the glycosidases on the metabolism of arabinogalactan-protein in Bifidobacteria
| Project/Area Number |
24580144
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Kagoshima University |
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Principal Investigator |
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| Research Collaborator |
SHIMOKAWA Michiko
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 糖質分解酵素 / プレバイオティクス / アラビノガラクタン-プロテイン / ビフィズス菌 / 腸内細菌 / Ⅱ型アラビノガラクタン / ガラクトース / アラビノース |
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| Outline of Final Research Achievements |
Type II arabinogalactan is a suitable carbohydrate source for Bifidobacterium longum, but the metabolic pathway has never been revealed. In this study, we cloned and characterized GH43 exo-β-1,3-galactanase (BLLJ_1840), GH30 β-1,6-galactanase (BLLJ_1841), and GH43 α-L-arabinofuranosidase (BLLJ_1854) from B. longum JCM1217. Furthermore, we revealed a degradation pathway for type II arabinogalactan in B. longum.
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| Free Research Field |
糖鎖工学、酵素工学、遺伝子工学
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