Application of in vitro C2C12 contractile model that mimics different types of exercise

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580147
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Toyo University
• Principal Investigator: NEDACHI Taku 東洋大学, 生命科学部, 教授 (50375200)
• Co-Investigator(Kenkyū-buntansha): KAWAGUCHI Hideo 東洋大学, 生命科学部, 教授 (50416921)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 骨格筋 / 運動 / 分泌タンパク質

Outline of Final Research Achievements

In this study, by utilizing C2C12 contractile model, we attempted to mimic different types of exercise, such as vigorous exercise, and analyzed exercise-type dependent changes in cellular response, such as production of myokines. Differentiated C2C12 myotubes were subjected to different voltage, pulse frequency, duration of electrical pulse stimulation (EPS) to induce contraction, thereby analyzed secreted proteins by membrane antibody arrays. Several novel myokines, which were induced by vigorous exercise, were successfully identified by this method, particularly, we found two myokines, including RANTES, were significantly reduced by vigorous exercise. In addition, we found exercise-dependent AMP kinase activation was sufficient for reducing secreted RANTES levels. In conclusion, we identified RANTES as a novel myokine that secretion is reduced by vigorous skeletal muscle cell contraction, and this reduction is mediated by AMP kinase pathway.

Free Research Field

応用生物化学