Development of controlling system of cell differentiation/life by proteolysis resistant cell-permeable protein

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580148
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Nihon University
• Principal Investigator: MASUHIRO Yoshikazu 日本大学, 生物資源科学部, 講師 (00336083)
• Co-Investigator(Kenkyū-buntansha): SEKI Taiichirou 日本大学, 生物資源科学部, 教授 (20187834) ; HANAZAWA Shigemasa 日本大学, 生物資源科学部, 教授 (60060258)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 分解耐性 / スタビロン / 膜透過性タンパク質 / 細胞分化制御 / 細胞機能調節

Outline of Final Research Achievements

I established the expression system of the proteolysis resistant cell-permeable differentiation control factor (Yamanaka Factor [Glis1, Sox2, Kof4, Oct4], RARα, Foxp3, Sox17, HNF3β ). These many factors showed good expression in Escherichia coli and maintained transcription activity. In addition, I found that a fusion position of the proteolysis-resistant motif stabilon was effective at all positions. Furthermore, unlike an old stabilon motif, I developed a proteolysis-resistant motif of the absent new sequence in the creatures.

Free Research Field

分子細胞生物学、応用生物科学