Elucidation of protection strategy for toxicity of D-amino acid in plant

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580151
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Kansai University
• Principal Investigator: OIKAWA Tadao 関西大学, 化学生命工学部, 教授 (80233005)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: D-アミノ酸 / アミノ酸ラセマーゼ / シロイヌナズナ / D-セリン / L-セリン

Outline of Final Research Achievements

We succeeded to construct the serine racemase gene-disruption mutant and overexpressed mutant of Arabidopsis thaliana. By using these mutants and wild-type strain of Arabidopsis thaliana, we evaluated the effects of various D- and L- amino acids on germination and growth of these strains. In case of D-serine, the serine racemase gene overexpressed mutant showed higher tolerant than other strains. This probably attributed to the D-serine degradation activity of serine rasemase. In case of L-serine, both serine racemase gene-disruption mutant and overexpressed mutant showed higher tolerant than wild-type strain.

Free Research Field

酵素科学