Cyclization mechanism analysis of terpene cyclases involved in C-C rearrangement

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580159
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: KAWAIDE Hiroshi 東京農工大学, (連合)農学研究科(研究院), 准教授 (20291916)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 生合成 / テルペノイド / ジテルペン / 環化酵素 / NMR

Outline of Final Research Achievements

We performed two subjects on the theses. (1) Functional analysis using isotopomers of substrate proposed a cyclization reaction mechanism of a diterpene produced by diterpene synthase from Aspergillus oryzae. Absolute configuration of the diterpene was determined by direct comparison with the same diterpene metabolite produced by another fungal metabolite. (2) Eight candidate genes encoding diterpene cyclases in Jatropha curcas were searched from our analysis data from the next generation sequencing and database published on the web. Functional analysis of the genes are in progress.
Related work was published on the Biochemical Journal.

Free Research Field

天然物化学