2014 Fiscal Year Final Research Report
Regulation of inflammatory responses by food-derived compounds
| Project/Area Number |
24580177
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 自然免疫 / Toll様受容体 / 食品成分 / 炎症 |
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| Outline of Final Research Achievements |
Toll-like receptors (TLRs) have been implicated in the pathogenesis of chronic inflammatory diseases. In this study, based on an extensive screening of vegetable extracts for TLR-inhibiting activity, we found cabbage extracts to be the richest sources of a TLR signaling inhibitor. We performed activity-guiding separation of the principal inhibitors and identified iberin from the cabbage. In addition, it was revealed that iberin specifically acted on the dimerization step of TLRs in the TLR signaling pathway. Moreover, we developed a novel probe combining an isothiocyanate-reactive group and an alkyne functionality for click chemistry and detected the probe bound to the TLRs in living cells, suggesting that iberin disrupts dimerization of the TLRs via covalent binding. These findings establish the TLR dimerization step as a target of food-derived anti-inflammatory compounds.
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| Free Research Field |
食品生化学
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