2014 Fiscal Year Final Research Report
Functional study of IL-5-producing cells in food allergy using GFP marking system
| Project/Area Number |
24580196
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Dokkyo Medical University |
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Principal Investigator |
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| Research Collaborator |
KOBAYASHI Ayano
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | アレルギー / IL-5 / IL-33 / 好酸球 / GFP / マーキング |
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| Outline of Final Research Achievements |
Eosinophils are multifunctional leukocytes involved in parasite elimination, evocation of allergic reactions, and regulation of adipose tissue. IL-5 is required for eosinophil survival; however, the in vivo mechanisms of eosinophil regulation are not fully understood. A transgenic (tg) mouse model with Il5 promoter-driven EGFP expression was established for detecting the IL-5-producing cells in vivo. The first part of the results demonstrate that innate lymphoid cells (ILCs) activate eosinophils in GAT. The blockage of IL-33R, on the other hand, did not impair EGFP+ ILC numbers but did impair eosinophil numbers in vivo. IL-33 was found to expand eosinophil numbers in CD90+ cell-depleted mice. The latter part of findings demonstrate that IL-33 directly activates eosinophils in GAT, and together with our other findings described above, our findings show that IL-33 has dual pathways via which it activates eosinophils in vivo: a direct activation pathway and a group 2 ILC-mediated pathway.
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| Free Research Field |
免疫学
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