Temporal and spatial behavior of Golgi bodies involved in the biosynthesis, secretion and modification of non-cellulosic polysaccharides of wood fiber secondary wall

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580243
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Wood science
• Research Institution: Kyoto University
• Principal Investigator: AWANO Tatsuya 京都大学, (連合)農学研究科(研究院), 助教 (40324660)
• Co-Investigator(Renkei-kenkyūsha): SUZUKI Shiro 京都大学, 生存圏研究所, 助教 (70437268) ; TAKATA Naoki 独立行政法人森林総合研究所, 森林バイオ研究センター, 主任研究員 (90605544)
• Research Collaborator: MELLEROWICZ Ewa J. ; TANAKA Ryo ; TAKAI Tomohiro
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ヘミセルロース / ゴルジ体 / ライブセルイメージング / グルコマンナン / キシラン / キシラン転移活性 / 木部繊維

Outline of Final Research Achievements

The localization of fluorescence protein-tagged glucomannan synthase was shown to be similar to that of Golgi marker protein by live-cell imaging of a rice protoplast. In a protoplast co-expressing these proteins, particles with individual fluorescence and those with merged fluorescence were found, suggesting that the level of each protein varies among different Golgi bodies. In transgenic poplar expressing fluorescence protein-tagged glucomannan synthase, moving particles were found in leaf epidermal cells and stem differentiating fiber cells, while unmoving particles were found in leaf mesophyll cells, suggesting that the behavior of Golgi body varies among cell types.
Xylanase was found in cell wall, whereas not found in Golgi body. Xylan endotransglycosylase activity was detected in proteins extracted from cell wall. In situ activity was specific to fiber cells, suggesting that the transglycosylation of xylan is essential for the structure and function of fiber secondary wall.

Free Research Field

樹木細胞学