Determination of causative agent of akoya oyster disease by combination of NGS-based shotgun metagenomic sequencing and DNA array technology.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580291
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General fisheries
• Research Institution: Fisheries Research Agency
• Principal Investigator: TOMOMASA Matsuyama 独立行政法人水産総合研究センター, 増養殖研究所, 研究員 (20372021)
• Co-Investigator(Kenkyū-buntansha): NAKAYASU Chihaya 水産総合研究センター, 本部, コーディネーター (00311225) ; YASUIKE Motoshige 水産総合研究センター, 中央水産研究所, 研究員 (20604820) ; FUJIWARA Atsushi 水産総合研究センター, 中央水産研究所, グループ長 (30443352) ; TAKANO Tomokazu 水産総合研究センター, 増養殖研究所, 研究員 (40533998) ; NAKAMURA Youji 水産総合研究センター, 中央水産研究所, 研究員 (90463182)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 赤変病 / メタゲノム / アコヤガイ

Outline of Final Research Achievements

We attempted to determine the causative agent of akoya oyster disease by combination of NGS-based metagenomics and DNA array technology.
Shotgun metagenomic sequences of hemolymph were obtained from diseased and healthy oyster. Common sequences in diseased and healthy oyster were subtracted from the sequences from diseased oyster, and remaining 76,542 sequences were analyzed by Blast homology search. A large portion of the sequence shows homology to eubacteria, but sequences homologous to the animal virus was rare. Candidate sequences of pathogen were narrowed to 1,890 by using DNA microarray that constructed from subtracted 76,542 sequences.

Free Research Field

魚病