Endocrine regulation of triacylglycerol-transporting molecules involved in species-specific lipid accumulation in fish

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580297
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Fisheries chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: KANEKO Gen 東京大学, 農学生命科学研究科, 助教 (30466809)
• Co-Investigator(Renkei-kenkyūsha): KONDO Hidehiro 東京海洋大学, 海洋科学技術研究科, 准教授 (20314635)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 脂質 / リパーゼ / 成長ホルモン / トラフグ / マダイ / ヒラメ

Outline of Final Research Achievements

In this project, we first determined lipid distribution patterns across 10 species by oil red O staining. The mRNA levels of lipoprotein lipase (LPL) were well associated with the lipid distribution patterns, suggesting that the species-specific lipid distribution was attributed to the differences in lipid incorporation to skeletal muscle. We also characterized hormone-sensitive lipase (HSL), which is involved in lipolysis under fasting conditions, in several fish species. Subsequent ex vivo experiments revealed that the expression of LPL and HSL were regulated by growth hormone. Taken together with the species-specific distribution patterns of growth hormone receptor, we proposed a hypothesis that sensitivity to this hormone is a major determinant of lipid distribution patterns in fish.

Free Research Field

水産化学