Efficient bioethanol production by the created yeast with saccharification activity of brown algae

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580299
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Fisheries chemistry
• Research Institution: Tokyo University of Marine Science and Technology
• Principal Investigator: URANO Naoto 東京海洋大学, 海洋科学技術研究科, 教授 (90262336)
• Co-Investigator(Kenkyū-buntansha): 石田 真巳 東京海洋大学, 大学院海洋科学技術研究科, 准教授 (80223006)
• Co-Investigator(Renkei-kenkyūsha): 西沢 正文 慶應大学, 医学部, 専任講師 (80223006)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 褐藻 / ラミナラン / ラミナラナーゼ / 糖化 / 酵母 / 細胞表層工学 / バイオエタノール / 未利用資源

Outline of Final Research Achievements

In this study, we aimed the creation of a yeast with saccharification activities of brown algae and the production of bioethanol from the disposed unuseful resources. Cold-active laminaranases were screened from coasts in order to be agreed the temperature for yeast cultivation with that for enzyme activity and Pesudoalteromonas haloplanktis LA was isolated from The Sagami Bay in Japan. Its laminaranase PhLam was purified and the gene was cloned in Esherichia coli. PhLam was the novel enzyme which had repeated sequence; X1, X2, and X3 in the downstream of catalytic domain and X module was essential for expression of the enzyme. Phlam gene was expressed on the cell surface of the yeast Saccharomyces cerevisiae. The recombinant yeast had laminaranase activity and directly produced bioethanol from laminaran.

Free Research Field

環境微生物化学