Polymorphism of adiponectin gene in Landrace and Meishan breeds may associate with backfat thickness in pigs

2015 Fiscal Year Final Research Report

• Project/Area Number: 24580417
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied animal science
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: Nakajima Ikuyo 国立研究開発法人農業・食品産業技術総合研究機構, 畜産研究部門・畜産物研究領域, 上級研究員 (60355063)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 畜産学 / 生理活性 / ブタ / 皮下脂肪 / アディポネクチン / 品種間比較

Outline of Final Research Achievements

In this study, polymorphism in porcine adiponectin was evaluated for associations with adiponectin multimer formation and backfat thickness between Landrace and Meishan breeds. A novel monoclonal antibody against porcine adiponectin was developed for use in Western blot and ELISA analysis. Although one SNP was located in coding region of Landrace and Meishan adiponectin genes which altered the protein sequence Val60Ile, there seemed to be no obvious differences in adiponectin multimers between these two breeds. Similar results were also obtained from the study by cultured cells transfected with cDNA encoding Landrace or Meishan adiponectin. Instead of the difference in multimeric patterns of adiponectin, the blood adiponectin concentration was significantly lower in high backfat Meishan pigs than Landrace pigs, which results were consistent with the mRNA levels of subcutaneous adipose tissue.

Free Research Field

ブタの脂肪組織発達