Function of vitamin B12 on neural cell differentiation and an insight on cognitive symptom.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580428
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Osaka Prefecture University
• Principal Investigator: TAKENAKA Shigeo 大阪府立大学, 生命環境科学研究科(系), 准教授 (10280067)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ビタミンB12 / 神経 / 分化 / レイチノイン酸

Outline of Final Research Achievements

Vitamin B12 (B12) is an essential nutrient and its serum content is correlated with progression of cognitive symptom as seen in Alzheimer’s and Parkinson’s disease. Here I examined effects of B12 metabolizing protein down regulation using RNA interference technique on differentiation of SH-SY5Y cell by retinoic acid to have an insight on the relation between the symptom and B12. The down regulation of the genes decreased the rate of differentiation without any significant change of metabolite profile. Mitochondrial dysfunction and oxidative stress which happen under B12 deficient condition were not observed in the cells. These indicate that B12 metabolism has a part in protective function on differentiation of neural cell in unknown pathway.

Free Research Field

獣医生化学、栄養学