Functional analyses on EphA2 in monocytes/macrophages

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580429
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Osaka Prefecture University
• Principal Investigator: OGAWA Kazushige 大阪府立大学, 生命環境科学研究科(系), 教授 (60231221)
• Co-Investigator(Renkei-kenkyūsha): ISHII Maki 大阪府立大学, 生命環境科学研究科(系), 客員研究員 (50415535)
• Research Collaborator: PASQUALE Elena Sfoanrd-Burnham Medical Research Institute (La Jolla, USA), 教授
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: EphA2 / ephrin-A1 / 単球 / マクロファージ / 血管内皮細胞

Outline of Final Research Achievements

EphA2 expression was regulated during the monocyte (Mo) differentiation, and EphA2 and ephrin-A1 expressions were regulated by TNFα in macrophages (Mφ) and vascular endothelial cells (EC). Adhesion to Matrigel coated surface in Mo, Mφ and the Mo/Mφ cell line J774.1 was regulated by ephrin-A and EphA activation. EphA2 activation regulated cell-cell adhesion and actin filament formation in EC. EphA signaling in J774.1 regulated transendothelial migration in vitro. Moreover, J774.1 cell infiltration into the red pulp of the mouse spleen was regulated by ephrin-A/EphA signaling in vivo. These results may indicate that EphA and ephrin-A regulate transendothelial migration of Mo/Mφ into inflamed tissues.

Free Research Field

獣医解剖学