2014 Fiscal Year Final Research Report
Role of Mitf, a tissue-specific transcription factor, in osteoclastogenesis and osteoclast function, and molecular mechanisms underlying Mitf-mediated osteoclastogenesis
| Project/Area Number |
24580437
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Azabu University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
FUNABA Masayuki 京都大学, 大学院農学研究科, 准教授 (40238655)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 破骨細胞分化 / 転写因子 / Mitf |
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| Outline of Final Research Achievements |
Treatment with RANKL induces osteoclastogenesis in murine and canine bone marrow cells as well as murine RAW264.7 cells. Analyses of Mitf isoform expression indicated that Mitf-E gene was induced within 24 hours after RANKL treatment in all examined cells. Knockdown of Mitf-E by its siRNA transfection inhibited RANKL-induced osteoclastogenesis. In mouse cells, co-treatment with TGF-β enhanced RANKL-induced osteoclastogenesis, whereas TGF-β inhibited canine osteoclastogenesis induced by RANKL.
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| Free Research Field |
分子生物学
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