Study on mechanism of contamination for chicken carcasses during processing and development of an effective method for decontamination of the organisms.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580447
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied veterinary science
• Research Institution: University of Miyazaki
• Principal Investigator: MISAWA NAOAKI 宮崎大学, 産業動物防疫リサーチセンター, 教授 (20229678)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: カンピロバクター / ブロイラー / 食中毒 / 微生物制御 / 付着因子 / 食鳥処理 / HACCP

Outline of Final Research Achievements

Transmission of Campylobacter to humans is considered to be consumption of chicken meat that has been contaminated during processing. In the present study, adhesion factors in both of Campylobacter and chicken skin have been examined. Mutants which lack cell surface materials were constructed by natural transformation-mediated allelic exchange. The mutagenesis studies clearly indicated that capsular polysaccharide expression on the cell surface played a major role in the attachment on skin surfaces. Then, the numbers of Campylobacter attached to the skin before and after treatment with sodium hydroxide were determined. In comparison with pretreated skin, the mean reductions obtained by the alkaline treatment exceeded the one log CFU per 10 g back skin, suggesting that adhesion factors on the skin may be extracted by this treatment. An adhesion factor showing about 100-150 KDa was detected by a two-dimensional SDS-PAGE and over-lay assay.

Free Research Field

獣医公衆衛生学