The role of Babesia gibsoni heat shock protein 90 on the proliferation and the drug resistance.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580456
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Clinical veterinary science
• Research Institution: Iwate University (2014); Hokkaido University (2012-2013)
• Principal Investigator: YAMASAKI Masahiro 岩手大学, 農学部, 教授 (40322846)
• Co-Investigator(Kenkyū-buntansha): OHTA Hiroshi 北海道大学, (連合)獣医学研究科, 講師 (50431333) ; TAKIGUCHI Mitsuyoshi 北海道大学, (連合)獣医学研究科, 教授 (70261336)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: バベシア症 / Babesia gibsoni / 熱ショックタンパク質90 / クローニング / リアルタイムRT-PCR / ウェスタンブロッティング / 熱ショックタンパク質90阻害薬

Outline of Final Research Achievements

In this study, Heat shock protein 90 (Hsp90) in Babesia gibsoni, a blood protozoa in dogs, was analyzed. The Babesia gibsoni heat shock protein 90 (BgHsp90) gene was cloned and sequenced. Moreover, anti recombinant BgHsp90 antibody was developed. Additionally, the expression of BgHsp90 was observed. As results, it was shown that BgHsp90 might contribute the proliferation of the parasites, and that the expression of BgHsp90 would be elevated when the parasites were exposed to the high temperature. Meanwhile, Hsp90 inhibitors could suppress the proliferation of B. gibsoni in vitro. This result suggested that the BgHsp90 should be one of the drug targets for the treatment of canine babesiosis.

Free Research Field

獣医内科学