2014 Fiscal Year Final Research Report
Investigation of the mechanism in the aldosterone regulation through the beta-adrenergic receptor
| Project/Area Number |
24580463
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Clinical veterinary science
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 循環器 / 線維化 / アルドステロン / ミネラルコルチコイド受容体 / エプレレノン / 交感神経 / β受容体 / コラーゲン |
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| Outline of Final Research Achievements |
In vivo study, the isoproterenol (ISO) increased cardiac fibrosis and expression of collagen-I mRNA/ protein in rat left ventricle. These responses were inhibited by the simultaneous administration of the aldosterone receptor inhibitor, eplerenone; EPL. In addition, phosphorylation of ERK1/2 induced by ISO was inhibited with the simultaneous administration of EPL. However, gene expression of aldosterone syntheses was neither detected in the left ventricle nor cardiac fibroblast.
In vitro study, the ISO increased expression of collagen-I and phosphorylation of ERK1/2 in the cardiac fibroblasts. ISO-induced these responses were inhibited by the simultaneous treatment of EPL.
These results suggested that the aldosterone receptor participates in the collagen-I synthesis through the adrenergic receptor in the rat left ventricle/cardiac fibroblast.
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| Free Research Field |
獣医内科学
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