Establishment of cartilage regenerative medicine using canine bone marrow stromal cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580465
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Clinical veterinary science
• Research Institution: Nihon University
• Principal Investigator: EDAMURA Kazuya 日本大学, 生物資源科学部, 講師 (80366624)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 再生医療 / 犬 / 軟骨 / 骨髄間質細胞 / 変形性関節症 / 幹細胞 / 動物

Outline of Final Research Achievements

The purpose of this study is to establish a radical therapy of osteoarthritis by the cartilage regeneration medicine using bone marrow stromal cells (BMSCs) in dogs. Canine BMSCs could differentiated into cartilage-like structure using chondrogenic differentiation medium. The proliferative capacity of canine BMSCs that were cultured with serum-free medium was superior to that with conventional medium. However, chondrogenic potency of canine BMSCs that were cultured with serum-free medium was controversial. In this study, the mebiol gel was most suitable scaffold for canine artificial cultured cartilage. Finally, minimum invasive and optimal arthroscopic procedures for cartilage regeneration medicine could be established. These all results can contribute to the clinical application of cartilage regeneration medicine in dogs.

Free Research Field

獣医外科学