Development of lactate-producing white-rot fungi from woody biomass

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580475
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Boundary agriculture
• Research Institution: Shizuoka University
• Principal Investigator: HIRAI Hirofumi 静岡大学, (連合)農学研究科(研究院), 教授 (70322138)
• Co-Investigator(Kenkyū-buntansha): KIMBARA Kazuhide 静岡大学, 工学部, 教授 (30225122)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 乳酸発酵 / 白色腐朽菌 / バイオリアクター

Outline of Final Research Achievements

We demonstrated the molecular breedings of lactate-producing fungi in　 Phanerochaete sordida YK-624. Lactate dehydrogenase (LDH) gene of Bifidobacterium longum was introduced in P. sordida YK-624, and 0.690 g/L lactate was detected in culture fluid inoculated with the most producing transformant. To improve the production of lactate, we constracted pyruvate decarboxylase gene-knock down plasmid. Both the LDH expression plasmid and the knock down plasmid were co-transformed into P. sordida YK-624, and 0.922 g/L lactate was detected in culture fluid inoculated with the most producing transformant. Moreover, we developed the bioreactor which lignin was effectively degraded by white-rot fungi under solid state fermentation.

Free Research Field

環境生化学