The evaluation of environmental contaminants using human breast carcinoma cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580485
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Boundary agriculture
• Research Institution: Tokoha University
• Principal Investigator: KURUTO Ryoko 常葉大学, 教育学部, 准教授 (50205217)
• Co-Investigator(Kenkyū-buntansha): OHURA Takeshi 名城大学, 農学部, 准教授 (60315851)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 環境汚染物質 / 多環芳香族炭化水素 / ハロゲン化 / エストロジェン / ゲニステイン / 遺伝子発現

Outline of Final Research Achievements

A variety of contaminants are present in the environment. Their effects were investigated using human breast carcinoma MCF-7 and MDA-MB-231 cells. The water samples from Tomoe River in Shizuoka city and Manyo pond in Tokoha University showed estrogenic and cytotoxic activity. Then we focused benzo[a]pyrene (BaP), one of polycyclic aromatic hydrocarbons, and its halogenated derivatives, ClBaP and BrBaP. They indicated estrogenic activity. The profile of gene expression of BaP was different from that of the natural estrogenic substances such as estrogen (E2) and genistein (Gen). Their combined effects were examined using MCF-7 cells. Exposure to 10-6 M BaP led to inhibit the proliferative effects of E2. Although 10-5 M BaP itself strongly enhanced cell proliferation, its effect was reduced by the addition of E2. Exposure to ClBaP or BrBaP enhanced the proliferative effects of E2. Gen inhibited the induction of CYP1A1 and IL-6 gene expression by BaP and BrBaP.

Free Research Field

細胞生物学