Analysis of suppression of Th2-type immune responses through innate immunity by glyco-polymer bearing plant antigenic N-glycans

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580494
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Okayama University
• Principal Investigator: MAEDA Megumi 岡山大学, 環境生命科学研究科, 助教 (20434988)
• Co-Investigator(Renkei-kenkyūsha): KIMURA Yoshinobu 岡山大学, 大学院環境生命科学研究科, 教授 (70195384) ; OTSUKI Takemi 川崎医科大学, 衛生学, 教授 (40160551)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: N-グリカン / 植物抗原性糖鎖 / スギ花粉アレルゲン / Th2免疫応答抑制 / 多量精製 / ルイスa抗原 / 糖鎖ポリマー / 樹状細胞

Outline of Final Research Achievements

The plant antigenic N-glycans from plant glycoproteins have suppressed Th2-type immune responses by Japanese cedar pollen allergen, suggesting that the plant antigenic N-glycans could become effective agents in therapy for patients with pollinosis. In this study, a new method with hydrophilic partitioning was developed to prepare the large-scale of Asn-glycopeptide bearing plant antigenic N-glycans from Ginkgo seeds. And then, plant antigenic N-glycans bearing Lewis a antigen(s) at the non-reducing terminal were found in submerged plants. Finally, the glycol-polymer bearing multivalent plant antigenic N-glycans was synthesized, and the differential inhibition of dendritic cells by the glycol-polymer was analyzed.

Free Research Field

農学