2014 Fiscal Year Final Research Report
Functional analysis of cytosolic free N-glycans involved in regulation of plant growth and application of the biofunction of free N-glycans
| Project/Area Number |
24580495
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied molecular and cellular biology
|
|---|
| Research Institution | Okayama University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Keywords | 植物糖タンパク質 / 植物糖鎖 / 遊離 N-グリカン / PNGase / ENGase / トランスジェニック植物 |
|---|
| Outline of Final Research Achievements |
In developing plant tissues, free N-glycan that are enzymatically liberated from glycoproteins occur ubiquitously at micromolar level. As a part of study to elucidate the physiological function(s) of these free N-glycans involved in plant development, we constructed knock-out or knock-down plants, in which the expressions of ENGase or PNGase genes were suppressed. The structural features of free N-glycans expressed in those transgenic plants were clearly distinguishable from those expressed in wild type plants. Although significant difference in phenotype between the transgenic plants and wild type was not observed, a specific difference in the fruit-ripening process was observed.
|
| Free Research Field |
糖鎖生物学
|
