Development and application of NMR-based metabolomics for a novel diagnostic analysis of chronic kidney disease and life-style related diseases

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590042
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Tohoku University
• Principal Investigator: FUJIWARA MASAKO 東北大学, 薬学研究科(研究院), 准教授 (10466534)
• Co-Investigator(Kenkyū-buntansha): TAKEUCHI Kazuhisa 東北大学, 大学院薬学研究科, 分野研究員 (40260426) ; TOTSHUNE Kazuhito 東北福祉大学, 総合福祉学部, 教授 (10217515)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 1H NMR / メタボロミクス / 生活習慣病 / 慢性腎臓病 / 乳酸 / 透析廃液 / ビオチン

Outline of Final Research Achievements

For development of new diagnostic analysis for chronic kidney disease, we applied NMR-based metabolomics of plasma and dialysate from patients undergoing hemodialysis (HD). To investigate metabolic responses to HD, we have determined levels of metabolites by nuclear magnetic resonance (1H NMR) spectroscopy of dialysate during HD. Unlike the response of creatinine, lactate, pyruvate and alanine exhibited increments at the middle time of HD. The time-course of changes in these metabolites had unique to each patient.
Painful muscle cramp is a common complication of hemodialysis (HD). Oral administration of biotin ameliorated significantly the muscle cramps of HD patients. Then we measured plasma biotin levels at baseline an avidin-competitive assay (ELISA) and LC/MS/MS methods, they revealed significantly higher in patients with cramp than those without cramps. HD patients accumulated biotin than healthy subjects because of their anuric conditions.

Free Research Field

物理系薬学