2014 Fiscal Year Final Research Report
Verification of the connector function of the transcription and splicing factor PQBP1
| Project/Area Number |
24590049
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | University of Toyama |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
OBITA Takayuki 富山大学, 大学院医学薬学研究部(薬学), 准教授 (30578696)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | タンパク質 / 天然変性蛋白質 / 解離定数 / 表面プラズモン共鳴 |
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| Outline of Final Research Achievements |
The transcription and splicing factor PQBP1 is composed of a small folded WW domain and a long disordered region. In the present study, we quantitatively analyzed the interaction of PQBP1 with spliceosomal proteins WBP11 and U5-15kD using surface plasmon resonance technique. In addition, we also demonstrated that PQBP1 simultaneously binds to WBP11 and U5-15kD. Our results suggest that PQBP1 acts as a connector protein in spliceosome.
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| Free Research Field |
物理系薬学
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