Redox regulation of metabolic processes and oxidative stress in high-fat diet-induced obese mice

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590052
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Kyushu University
• Principal Investigator: YAMATO Mayumi 九州大学, 学内共同利用施設等, 准教授 (30380695)
• Co-Investigator(Renkei-kenkyūsha): YAMADA Kenichi 九州大学, 薬学研究院 (60346806)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 酸化ストレス / 肥満 / エネルギー代謝 / レドックス制御

Outline of Final Research Achievements

Obesity is a condition of well-developed adipose tissue, which causes functional abnormalities of energy metabolism. Continuous conversion of energy is controlled by reduction-oxidation (redox) processes. Oxidized and reduced nicotinamide adenine dinucleotide (NAD+ and NADH) are an important redox couple for energy metabolism. The ratio of NAD+/NADH has been implicated in oxidative stress. 4-Hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl (tempol) is a redox-cycling nitroxyl radical that promotes the scavenging of several reactive oxygen species, and is reduced simultaneously to the hydroxylamine by NADH. Tempol is also involved in NAD+ production in the ascorbic acid-glutathione redox cycle. We utilized the chemical properties of tempol to clarify the effect of antioxidants and NAD+/NADH modulators on metabolic imbalance in obese mice. Increases in the NAD+/NADH ratio by tempol ameliorated metabolic imbalance in combination with dietary improvement.

Free Research Field

生物物理化学