Development of technique for higher order structure of glycoprotein by hydrogen/deuterium exchange mass spectrometry

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590069
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: National Institute of Health Sciences
• Principal Investigator: HASHII Noritaka 国立医薬品食品衛生研究所, 生物薬品部, 室長 (20425672)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 水素重水素交換／質量分析 / 糖タンパク質 / 高次構造 / 抗TNF-α抗体

Outline of Final Research Achievements

In this study, as part of development of technique for higher order structure of glycoprotein, structural analysis of the anti-TNF-α agents/human soluble TNF-α interaction was performed by hydrogen deuterium exchange/mass spectrometry (HDX/MS). HDX/MS of the TNF-α with or without anti-TNF-α agents was revealed that the 4 agents protected common internal regions of TNF-α. This result suggested that anti-TNF-α agents exhibit pharmaceutical effects via common structural changes of TNF-α. Additionally, characteristic epitopes of these anti-TNF-α agents were also identified by HDX/MS analysis.

Free Research Field

分析化学