Role of V-ATPase in cellular ion environment and vesicle trafficking

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590086
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Iwate Medical University
• Principal Investigator: NAKANISHI Mayumi 岩手医科大学, 薬学部, 准教授 (20270506)
• Co-Investigator(Renkei-kenkyūsha): GOTO Naomi (MATSUMOTO Naomi) 岩手医科大学, 薬学部, 助教 (80403971)
• Research Collaborator: FUTAI Masamitsu 岩手医科大学, 薬学部, 教授 (50012646) ; OKAMOTO Haruko 岩手医科大学, 薬学部, 特任講師 (40552899)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: プロトンポンプ / 酸性環境 / 破骨細胞 / 小胞輸送 / 一分子観察 / ナノモーター / 骨代謝

Outline of Final Research Achievements

During differentiation into osteoclasts, lysosome moves to and fuses with plasmamembrane to secrete lysosomal enzymes necessary for bone resorption. We found that a3 isoform of V-ATPase on lysosomal membrane binds specifically to a GTP-binding protein that regulates vesicle trafficking along microtubules. The a3 showed stronger binding to inactive form of the factor than to active form. However, the factor should be active form to transfer lysosome to plasmamembrane. Furthermore, the factor was not located on lysosome in osteoclasts from a3 gene knockout mice. These results indicate that a3 recruits inactive form of the vesicle trafficking factor into lysosomal membrane.

Free Research Field

生化学、細胞生物学、生物物理学