2014 Fiscal Year Final Research Report
Identification of mammalian aspartate racemase
| Project/Area Number |
24590090
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Biological pharmacy
|
|---|
| Research Institution | Kitasato University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Keywords | D-アスパラギン酸 / ラセマーゼ / D－アミノ酸 / 組換えタンパク質 / siRNA |
|---|
| Outline of Final Research Achievements |
The identification of D-aspartate (D-Asp) synthesizing enzyme, mammalian Asp racemase was conducted. All trials were unsuccessful to prepare the recombinant proteins with the candidate genes in E. coli cells, since the proteins were all recovered in the insoluble fractions. Therefore, the issue was addressed whether the D-Asp synthesis was influenced by small interference RNA (siRNA) of the candidate genes. The suppression of a gene (Got1l1), a candidate gene reported by an American research group had no influence on the production of D-Asp in the cultured mammalian cells those can synthesize D-Asp. The gene Got1l1 is presumably uninvolved in the biosynthesis of D-Asp in the mammalian cells.
|
| Free Research Field |
生物系薬学
|
