2014 Fiscal Year Final Research Report
Sensitive measurement of oxidized phospholipids as markers of oxidative stress and diseases.
| Project/Area Number |
24590094
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Showa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KATO Rina 昭和大学, 薬学部, 助教 (30392400)
OBAMA Takashi 昭和大学, 薬学部, 助教 (60395647)
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| Research Collaborator |
NOGUCHI Emiko
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 酸化LDL / 動脈硬化症 / 糖尿病 / 歯周病 / ELISA / 酸化リン脂質 / 酸化ストレス / リポタンパク質 |
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| Outline of Final Research Achievements |
Oxidized low-density lipoprotein (oxLDL) present in vivo, which is involved in atherogenesis, has not been well characterized. In this study, we introduced LC-MS/MS procedure to quantify various oxidized phosphatidylcholine (oxPC) and lysoPC species with high sensitivity. With this technique we elucidated molecular species of oxPC and lysoPC produced during LDL oxidation process. In addition, we successfully separated in vivo oxLDL from human plasma for the first time using an anion-exchange column and a monoclonal antibody against oxidized PC. The in vivo oxLDL was revealed to be a particle with anionic charges and modification with oxPC. oxLDL could potentially be a marker to evaluate oxidative stresses in systemic diseases and/or local tissues.
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| Free Research Field |
生化学
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