2014 Fiscal Year Final Research Report
Molecular basis of the avian memory formation regulated by cytoskelton binding protein
| Project/Area Number |
24590096
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Teikyo University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
AOKI Naoya 帝京大学, 薬学部, 助教 (60525334)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 生化学 / 行動生化学 / 分子生物学 / 記憶 / 刻印付け |
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| Outline of Final Research Achievements |
Birds have a diverse behavioral repertoire and are thus ideal models for experimental analyses of neural and behavioral plasticity. The domestic chick has been intensively studied as a model for filial imprinting. To elucidate the molecular processes underlying the neural mechanism of filial imprinting in newly-hatched chicks more extensively, we previously conducted a cDNA microarray and the quantitative RT-PCR study. One of the genes was microtubule-associated protein 2 (MAP2), which is involved in cytoskeletal organization. Here, we used in vivo gene-transfer technique and showed that transfected microRNA vectors preferentially silence exogenous DNA expression in neuronal cells. Using this system, the up-regulation of MAP2 accompanying filial imprinting was suppressed in vivo, which impaired the filial imprinting in chicks. Our results suggest that the regulation of MAP2 expression is required for filial imprinting.
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| Free Research Field |
生化学
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