Development of an in vitro allergenicity test metod for drugs considered metabolic activation

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590157
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Drug development chemistry
• Research Institution: Tokyo University of Marine Science and Technology (2013-2014); National Institute of Health Sciences (2012)
• Principal Investigator: KUROSE Kouichi 東京海洋大学, 海洋科学技術研究科, 教授 (30280754)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: インビトロ試験法 / 薬物アレルギー / 感作性

Outline of Final Research Achievements

Using human monocytic cell line THP-1, we developed a simple and high-throughput method to evaluate drug allergenicity in vitro, which is utilizing real-time RT-PCR. In addition, we established the cell lines, from THP-1, stably expressing a representative drug metabolizing enzyme CYP3A4, which is expected to be utilized for our new method above mentioned.

Free Research Field

医歯薬学