2014 Fiscal Year Final Research Report
Development of an in vitro allergenicity test metod for drugs considered metabolic activation
Project/Area Number |
24590157
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Drug development chemistry
|
Research Institution | Tokyo University of Marine Science and Technology (2013-2014) National Institute of Health Sciences (2012) |
Principal Investigator |
KUROSE Kouichi 東京海洋大学, 海洋科学技術研究科, 教授 (30280754)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Keywords | インビトロ試験法 / 薬物アレルギー / 感作性 |
Outline of Final Research Achievements |
Using human monocytic cell line THP-1, we developed a simple and high-throughput method to evaluate drug allergenicity in vitro, which is utilizing real-time RT-PCR. In addition, we established the cell lines, from THP-1, stably expressing a representative drug metabolizing enzyme CYP3A4, which is expected to be utilized for our new method above mentioned.
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Free Research Field |
医歯薬学
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