2014 Fiscal Year Final Research Report
Activation of cellular signal transduction pathways in protective response to an atmospheric electrophile, 1,4-naphthoquinone
| Project/Area Number |
24590159
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental pharmacy
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KUMAGAI Yoshito 筑波大学, 医学医療系, 教授 (00250100)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 1,4-ナフトキノン / 親電子物質 / ストレス応答 / HSP/HSF1 / Keap1/Nrf2 / PTP1B/EGFR |
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| Outline of Final Research Achievements |
1,4-Naphthoquinone (1,4-NQ), an environmental electrophile, is capable of covalently binding to cellular proteins, which has been recognized to be associated with not only its toxicity but also the activation of cellular signal transduction pathways for cellular protection against reactive chemicals. In this study, we prepared specific anti-1,4-NQ antibody and found that 1,4-NQ-mediated covalent modification of heat shock protein 90 (HSP90) causes activation of heat shock factor-1 (HSF1), resulting in protection against 1,4-NQ toxicity in A431 cells. Furthermore, Keap1/Nrf2 and PTP1B/EGFR pathways as well as HSP90/HSF1 pathway also found to play a role in protective response to 1,4-NQ toxicity.
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| Free Research Field |
毒性学
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