2014 Fiscal Year Final Research Report
Study to develop novel anti-HIV strategy targeting to weak point of HIV life cycle
| Project/Area Number |
24590161
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental pharmacy
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| Research Institution | Kumamoto University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | HIV |
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| Outline of Final Research Achievements |
In this study, we investigated NMT1-binding proteins using co-immunoprecipitation and mass spectrometry. Interestingly, hRNP A2/B1 was found to be associated with NMT1, which was not mediated by RNA. It was also suggested that hnRNP A2/B1 contributes to the formation of complexes of high molecular weights involving NMT1. Knockdown of hnRNP A2/B1 resulted in the enhancement of viral replication with an increase in the expression level of viral RNA in HIV-1-producing cells. On the other hand, knockdown of NMT1 resulted in attenuation of the viral replication with the decrease in the expression level of viral RNA in HIV-1-producing cells. Additionally, overexpression of NMT1 induced the enhancement of viral replication with the increase in the expression level of the viral RNA. These findings suggest that both NMT1 and hnRNP A2/B1 take part in the regulation of HIV-1 RNA expression through their mutual reverse effects on the viral RNA expression in HIV-1-producing cells.
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| Free Research Field |
医歯薬学
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