2015 Fiscal Year Final Research Report
Comparative genome analysis of nontuberculous mycobacteria and its clinical application
| Project/Area Number |
24590164
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental pharmacy
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| Research Institution | Meijo University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | Mycobacterium avium / 肺M. avium症 / ゲノム解析 / 新規プラスミド / 感染様式 |
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| Outline of Final Research Achievements |
To explore the bacterial factors that affect the pathological state of disease caused by Mycobacterium avium, we determined the complete genome sequence of strain TH135 isolated from a patient with pulmonary M. avium disease, and further demonstrated the presence of a novel plasmid, pMAH135. This plasmid consists of 194,711 nucleotides and has 164 coding sequences, some of which encode proteins involved in the pathogenicity of mycobacteria and their resistance to antimicrobial agents. The screening of M. avium isolates from humans and pigs for genes located on pMAH135 revealed that these genes are more commonly detected in isolates from patients with pulmonary M. avium disease than in HIV-positive patients. However, the genes were almost entirely absent in isolates from pigs, suggesting that pMAH135 influences not only the pathological manifestations of M. avium disease, but also the host specificity.
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| Free Research Field |
医歯薬学
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