Development and application of order-made dignosis using omics-integrated analysis of chronic kidney disease and hemodialysis patients

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590178
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Medical pharmacy
• Research Institution: Tohoku University
• Principal Investigator: TAKEUCHI KAZUHISA 東北大学, 薬学研究科(研究院), 分野研究員 (40260426)
• Co-Investigator(Kenkyū-buntansha): FUJIWARA Masako 東北大学, 大学院薬学研究科, 准教授 (10466534) ; TOTSUNE Kazuhito 東北福祉大学, 総合福祉学科, 教授 (10217515) ; SATO Emiko 東北大学, 大学院薬学研究科, 助教 (20466543)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 1H NMR / メタボロミクス / CKD / 透析患者 / 透析廃液 / フェノーム / 透析痙攣 / ビオチン

Outline of Final Research Achievements

For development of order-made diagnostis for chronic kidney disease and hemodailysis (HD) patients, we applied omocs-integrated analysis of plasma and dialysate from HD patients. To investigate metabolic responses to HD, we have determined levels of metabolites by nuclear magnetic resonance (1H NMR) spectroscopy of dialysate during HD. Unlike the response of creatinine, lactate, pyruvate and alanine exhibited increments at the middle time of HD. The time-course of changes in these metabolites was unique to each patient.
Muscle cramp is a common complications of HD. We defined score of the cramps and measured the effects of oral administration of biotin to cramps. We measured plasma biotin levels of the patients before prescribed biotin by ELISA methods, and found they were dependent on the efficacies of prescribed biotin. The fact of fenome (cramp scores) and metabolome (biotin levels) were correlated may provide information to the order-made diagnosis of HD patients.

Free Research Field

医療系薬学