2014 Fiscal Year Final Research Report
Development of safety vaccine using spleen- and dendritice cells-targeted nano device
| Project/Area Number |
24590198
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Medical pharmacy
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SASAKI Hitoshi 長崎大学, 病院(医学系), 教授 (00170689)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | ワクチン / ナノ材料 / 癌 / 感染症 |
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| Outline of Final Research Achievements |
In this study, we developed the novel melanoma vaccine and malaria vaccine using spleen-targeted nano-device. We used pUb-M which expresses melanoma-related antigen (gp100 and tyrosinase-related protein 2 (TRP2)) as melanoma DNA vaccine. We successfully prepared the stable nanoparticles (pUb-M/PEI/γ-PGA complexes) by optimization of the mix ratio. The pUb-M/PEI/γ-PGA complex significantly inhibited the growth and metastasis of a melanoma cell line, B16-F10 cells, and improved the survival time of metastasis model mice. For the development of malaria DNA nano-vaccine, PyGPI8p-transamidase-related protein (PyTAM) was selected as a possible candidate vaccine antigen. The pyTAM/PEI/γ-PGA complexes suppressed parasitemia and prolonged survival.
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| Free Research Field |
医歯薬学
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