M-protein binding oligosaccharides as a candidate of biomarker for multiple myeloma

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590209
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Medical pharmacy
• Research Institution: Bunkyo Gakuin University
• Principal Investigator: IIJIMA Shiro 文京学院大学, 保健医療技術学部, 教授 (30222798)
• Co-Investigator(Renkei-kenkyūsha): HATTORI Yutaka 慶應義塾大学, 薬学部, 教授 (20189575)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 多発性骨髄腫 / Mタンパク / 糖鎖

Outline of Final Research Achievements

To develop a new biomarker for multiple myeloma (MM), the reactivity of L chain-binding oligosaccharides was determined using serum sample and MM cell lines. L chain-binding oligosaccharides derived from MM patients with complication showed high reactivity with Con A lectin. In MM cell lines, lectin reactivity of L chain-binding oligosaccharides was correlated with that of sugar chain on the cell surface, and sugar transferase GnT-V that is involved in tumor metastasis was expressed in the cell lines. Further, IL-6-dependent expression of sialic acid-binding oligosaccharide on cell surface was observed. These results suggested that L chain-binding oligosaccharides reflects sugar chain on MM cell surface, and can be a candidate as biomarker of MM pathological condition.

Free Research Field

病態生化学