2014 Fiscal Year Final Research Report
Roles of Rspo3 during cushion tissue formation in the chick embryonic heart
| Project/Area Number |
24590238
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
General anatomy (including Histology/Embryology)
|
|---|
| Research Institution | Osaka City University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Keywords | 心臓 / 心内膜床 |
|---|
| Outline of Final Research Achievements |
Role of Rspo3 was examined in the endothelial-mesenchymal transformation (EMT) during cushion tissue formation using chick embryonic hearts. First, in situ hybridization showed Rspo3 mRNA was localized during the cushion tissue. Second, in vitro assay for cushion tissue formation showed that the administration of Rspo3, Rspo3+BMP2, and Rspo3+BMP2+wnt11 did not induce cell migration and invasion. Third, chick Rspo1, -2 and -4, genes were cloned and their expression patterns were examined during heart development. These results show that Rspo2 mRNA was expressed in the embryonic heart, but Rspo1 and 4 were not detected.
|
| Free Research Field |
発生学
|
