2014 Fiscal Year Final Research Report
Functional and morphological analyses of the cell polarity in developing terminal Schwann cells with special reference to caveolae and primary cilia.
| Project/Area Number |
24590247
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 感覚神経終末 / 終末シュワン細胞 / シュワン前駆細胞 / カベオラ / 一次線毛 / カルシウム画像 / アデノシン５’三燐酸 |
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| Outline of Final Research Achievements |
The unipolar, terminal Schwann cells in the cutaneous sensory devices have a round body and a long process, with the latter branching into glial lamellae. These cells, stellate Schwann-like cells and morphological intermediates between them were examined by immunohistochemistry and electron microscopy of vibrissal lanceolate sensory endings in young rats with special reference to the caveolae and the primary cilium that govern the cell polarity. Purinergically-evoked Ca signals were analyzed in the cells by confocal microscopy. Primary cilia of the stellate cells projected from a central concavity of the nucleus facing the follicular basement membrane, with many caveolae opening into the ciliary pocket. Evoked signals originated from the main body of the cells. Only rudimentary primary cilia occurred at the base of the main process in the intermediate and the unipolar cells, with caveolae accumulated in the lamellae. Ca responses were initiated at individual lamellae in these cells.
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| Free Research Field |
細胞生物学
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