2014 Fiscal Year Final Research Report
Analysis on changes in the molecular structure of glomerulus accompanying with diabetic nephropathy
| Project/Area Number |
24590260
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Kyorin University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 糖尿病 / ヘキソサミン代謝 / 糸球体 / O-GlcNAc / 糖尿病性腎症 / 糖転移酵素 / 基底膜 / グライコプロテオミクス |
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| Outline of Final Research Achievements |
It is well known that the changes in morphology of the glomerular epithelium, endothelium, basement membrane and mesangium occur in the diabetic nephropathy. However, the change of these structures at the molecular level is not examined yet. In this study to elucidate the etiology of diabetic nephropathy the change of the molecular structure of glomerulus was examined by the proteomics of O-GlcNAc-modified proteins from isolated glomeruli and immunohistochemistry. It was revealed that in the diabetic glomerulus some proteins including synaptopodin which was expressed specifically in the glomerular epithelial cells increased in O-GlcNAc-modification, and the localization of the O-GlcNAc-modified proteins changed.
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| Free Research Field |
医歯薬学
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