Regulation of hematopoiesis by bone marrow stromal cells in three-dimensional envirnment

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590262
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Nihon University
• Principal Investigator: AIZAWA Shin 日本大学, 医学部, 教授 (30202443)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 細胞分化・組織形成 / 造血微小環境 / 骨髄ストローマ細胞 / 三次元培養 / 造血因子

Outline of Final Research Achievements

The proliferation and differentiation of hematopoietic stem cells are regulated by bone marrow microenvironment. Stromal cells, as distinguished from hematopoietic cells, are an essential compartment of this microenvironment. In this study, the stromal cells are shown to regulate the proliferation and differentiation of hematopoietic cells through the mechanism by producing various hematopoietic regulatory factors. Further, a novel in vitro three-dimensional (3D) hematopoietic culture system was developed. In this culture system, stromal cells in 3D culture are stable and regulate hematopoietic cell proliferation and differentiation “quietly” during the culture, resulting in the presence of stable, resting hematopoietic stem cells in 3D culture.

Free Research Field

造血組織