Molecular physiological studies on functional differences among isoforms of ClC-3

2015 Fiscal Year Final Research Report

• Project/Area Number: 24590291
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General physiology
• Research Institution: National Institute for Physiological Sciences
• Principal Investigator: Okada Toshiaki 生理学研究所, 細胞器官研究系, 特任准教授 (00373283)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: イオンチャネル / 電気生理 / 分子クローニング

Outline of Final Research Achievements

ClC-3, a member of the Cl-channel/transpoter family, is predicted to have six isoforms, ClC-3a to -3f, with distinct N- and C-terminal amino acid sequences, however amino acid sequences of ClC-3d was derived from genomic sequence, but there was no experimental evidence for mRNAs. We recently cloned a cDNA containing the full coding region of ClC-3d. In this study, we analyzed the channel properties of ClC-3d by whole-cell patch-clamp recordings and identified basic properties of this channel. We identified that ClC-3d, like ClC- 3a, mediates Cd2+-sensitive outwardly rectifying anion currents and that ClC-3d is distinct from the molecular entities of acid- and volume-sensitive anion channels. Electrophysiological properties of ClC-3 have long been investigated but are still unclear. Our results may help elucidate them.

Free Research Field

生理学